A New Chapter in Histology
Histology is the gold standard for cellular resolution histopathological analysis. However, tissue morphology and density are irreversibly changed by the preparation steps, which include chemical fixation, dehydration, embedding, staining, and physical slicing. These manipulations make it difficult to draw conclusions about living tissues. In two recent publications, Griffin Rodgers and Bert Müller have provided convincing evidence that virtual histology based on X-ray imaging can overcome these limitations.
- Griffin Rodgers & Bert Müller, A New Context for Virtual Histology: 3D X-Ray Microimaging of Mouse Brain During Paraffin Embedding, SOLEIL Highlights 2021, pp. 48-49
- G. Rodgers, C. Tanner, G. Schulz, A. Migga, W. Kuo, C. Bikis, M. Scheel, V. Kurtcuoglu, T. Weitkamp, B. Müller
- Virtual histology of an entire mouse brain from formalin fixation to paraffin embedding. Part 2: Volumetric strain fields and local contrast changes; Journal of Neuroscience Methods 365 (2022) 109385; doi:10.1016/j.jneumeth.2021.109385
- G. Rodgers, W. Kuo, G. Schulz, M. Scheel, A. Migga, C. Bikis, C. Tanner, V. Kurtcuoglu, T. Weitkamp, B. Müller; Virtual histology of an entire mouse brain from formalin fixation to paraffin embedding. Part 1: Data acquisition, anatomical feature segmentation, tracking global volume and density changes; Journal of Neuroscience Methods 364 (2021) 109354; doi:10.1016/j.jneumeth.2021.109354
- Biomaterials Science Center